Rapid Method of Determining Factors Limiting Bacterial Growth in Soil

Rapid method of determining factors limiting bacterial growth in soil.

A technique to determine which nutrients limit bacterial growth in soil was developed. The method was based on measuring the thymidine incorporation rate of bacteria after the addition of C, N, and P in different combinations to soil samples. First, the thymidine incorporation method was tested in two different soils: an agricultural soil and a forest humus soil. Carbon (as glucose) was found t...

Factors Limiting Bacterial Growth

Simple assumptions have led to equations by which the latent period in multiplication and the bacterial numbers expected at any time during the phase of rapid growth may be predicted. Experimental data obtained under rather diverse conditions have given satisfactory agreement with calculated values. Since the mathematical expressions contain no arbitrary constants, more than accidental signific...

Rapid measurement of soil water by gravimetric method

Phosphate and soil binding: factors limiting bacterial degradation of ionic phosphorus-containing pesticide metabolites.

Soils that had a high binding capacity for inorganic orthophosphate (Pi) had reduced capacities to bind ionic alkyl phosphorus compounds. Only ionic methylphosphonate (MPn) and ionic phenylphosphonate exhibited moderate binding. Pseudomonas testosteroni used either MPn or Pi as a sole phosphorus source and exhibited diauxic utilization of MPn and Pi. The utilization of MPn was suppressed in the...

Measurement of bacterial growth rates in soil

Measurements of the average growth rates of soil bacteria from a soil under three treatments; a conventional high input corn soybean rotation, a reversion to a native successional community (4 years) and a never ploughed grassland, were made by two methods. The specific rates of synthesis of bacterial DNA were determined from the specific activities of the DNA precursor, deoxythymidine triphosp...